tris tricine criterion gel Search Results


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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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anti exse antibody  (Santa Cruz Biotechnology)
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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tris tricine sds page  (Thermo Fisher)
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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sodium dodecyl sulfate-polyacrylamide gel 4–20% tris-tricine gel  (Cosmo Bio USA)
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Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and <t>Western</t> <t>blotting.</t> A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient <t>Tricine</t> gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).
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Image Search Results


Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and Western blotting. A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient Tricine gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).

Journal: The American journal of pathology

Article Title: Inhibition of Early Growth Response 1 in the Hippocampus Alleviates Neuropathology and Improves Cognition in an Alzheimer Model with Plaques and Tangles.

doi: 10.1016/j.ajpath.2017.04.018

Figure Lengend Snippet: Figure 7 Inhibition of Egr-1 by shRNA reduces Ab pathology in the hippocampus of 3xTg-AD mice. The hippocampus and cortex of 3xTg-AD and nTg mice receiving shRNA-Ctl and shRNA-Egr-1 were analyzed by immunohistochemistry and Western blotting. A: Immunohistochemistry. Representative confocal images of hippocampus and cortex of 3xTg-AD and nTg mice, showing plaques. DAPI (blue) stained brain slices were counterimmunostained with human Ab42 antibody. Abundant Ab42-positive plaques are seen in both the hippocampus and the cortex of 3xTg-AD mice. A significant reduction in plaques is observed in the hippocampus of 3xTg-AD receiving shRNA-Egr-1 when compared to 3xTg-AD hippocampus receiving shRNA-Ctl. Quantification of immunohistochemical staining was performed using Spectrum Analysis algorithm package and ImageScope analysis software version 11.2 (Aperio Technologies). Data are expressed as the area of hippocampus or cortex occupied by Ab immunoreactivity. One-way analysis of variance and Newman-Keuls post hoc analysis were performed. B: Western blot analysis. Samples were separated on 4% to 20% gradient Tricine gel (Invitrogen). Western blotting was performed using anti-Ab40 or anti-Ab42 antibody. t-Test was performed. Data are expressed as means SEM. n Z 12 randomly selected micrographs from 12 animals in each genotype (A); n Z 6 per group (A); n Z 12 animals per genotype (B); n Z 6 per group (B). **P < 0.01. Scale bar Z 50 mm (A).

Article Snippet: For Western blotting, samples were separated on 10% to 20% Tricine gel (Invitrogen) and probed against either mouse or human anti-Ab40 or anti-Ab42 antibody.

Techniques: Inhibition, shRNA, Immunohistochemistry, Western Blot, Staining, Immunohistochemical staining, Software